Post extraction cellular viability of pulpal tissue

• Author Details:   
• A Sharma ^*
• R Saha
• P. B. Sood
• S Upadhyaya
• A Diwaker
• S Patanjali

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Aims & Objectives: The aim of this study was toassess theviability of dental pulp cells atdifferent intervals so that the clinical viable time of pulpal cells can be determined before pulp extirpation after traumatic injuries.

Materials & Methods: After ethical approval and with informed consent the pulp tissue samples were collected from 12 subjects meeting the inclusion and exclusion criteria. The sample was immediately transported to the laboratory for processing which included washing of the sample, mincing and enzymatic digestion followed by incubation and review for observation of growth.

Results & Conclusion: A total of twelve samples were processed out of which two samples showed positive growth of cells. The cells appeared to be mesenchymal cells and morphologically resembled fibroblast indicating the possibility that they are derived from dental pulp and are different from microorganisms. It can be concluded that the tissue can be kept viable for a certain period of time, post extraction, but there are stringent requirements of a completely sterile environment.

Keywords: Dentalpulp, Fibroblasts, Sterilization, Viability